Ethidium Bromide, Loading Buffer & DNA Ladder: Visualizing DNA and Determining its Size - Video | Study.com
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The following picture shows the ethidium bromidestained bands revealed by gel electrophoresis of two different DNA samples digested with two different restriction enzymes. One of the DNAs is human genomic DNA, the other is the small genome of a bacteriophage (bacterial virus) that infects E. coli cells. One of the restriction enzymes is E c o RI(5^' G^∧ A ATTC 3^') ; the other is HpaII(5^' C^∧ CGG 3^') . For each of the four lanes on the gel (A-D), identify which of the two DNA samples was analy
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Ethidium bromide is a compound commonly used to visualize DNA during gel electrophoresis, though its use has been phased out due to toxicity and mutagenic concerns. This compound stains DNA by intercalating. Explain what this means in the context of DNA base pairs.
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SOLVED: What is ethidium bromide (EtBr) ?Select one Itis used t0 determine DNA directly by spectroscopy It is reagent that convert DNA's 2 deoxyribose to furfural then [cacts with dark blue color that absorbs 600 nm diphenylamine t0 produce c It is a fluorescence dye that is used detect DNA by binding adjacent buse pairs absorb UV at 300 nm emits at 590n0ncatalyzed removal of purine base fTom nucletc acid It Leacts with aldehyde 0t Alleracid the RCHCHO formn to produce strongly fluorescetf c
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