Single-stranded oligodeoxyribonucleotide (ssODN) gene editing has emerged as a promising therapeutic strategy. However, further improvements in efficiency are desired for practical application. The ...

Prime editing enables precise and efficient genome editing, but its efficacy is hindered by pegRNA’s 3’ extension, forming secondary structures due to high complementarity with the protospacer. The ...

Precision gene editing is crucial for treating genetic diseases, as it enables targeted correction of specific mutations. A Korean research team has become the first in the world to significantly ...

Prime editing can potentially repair the vast majority of known disease-causing human mutations, but the technology, first developed in 2019, has not yet been widely used in the body, or in vivo, to ...

While CRISPR-mediated gene editing has led to powerful advances across biology, medicine, and agriculture, challenges persist in optimizing the editing efficiency of enzymes, such as the widely used ...

Toward the goal of efficient editing of farm animals for genetic improvements, this study explores an upgraded form of nuclease PE (uPEn). Based on an initial demonstration of its success in editing ...

Gene editing in plants remains challenging, with the traditional non-homologous end-joining (cNHEJ) repair pathway often hindering precision. In this study, researchers advanced CRISPR-Cas-based gene ...